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lightcycler 480 probes master kit  (Roche)


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    Structured Review

    Roche lightcycler 480 probes master kit
    Lightcycler 480 Probes Master Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+480+probes+master+kit/LightCycler+480+System/pmc13040562-155-8-13
    Average 99 stars, based on 6 article reviews
    lightcycler 480 probes master kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Exploratory Deep Phenotyping of Early Antidepressant Treatment Effects in a Longitudinal, Double-Blind, Randomized, Controlled, Parallel-Group Pre-Post Design – a Study Protocol from Research Consortium OptiMD
    Article Snippet: .. Concentration of MgCl 2 and annealing temperature had been optimized for these three sets of PCR primers and provided best results for 4 mM MgCl 2 and 60° C. A qPCR mixture with 20 μl final reaction volume was prepared by using LightCycler 480 Probes Master kit (Roche Diagnostics, Mannheim, Germany, catalog no. 04707494001) which provides a hot-start reaction mix with FastStart Taq DNA Polymerase designed specifically for detecting DNA targets with hydrolysis probes during qPCR with the LightCycler 480 II system. ..

    Polymerase Chain Reaction:

    Article Title: Exploratory Deep Phenotyping of Early Antidepressant Treatment Effects in a Longitudinal, Double-Blind, Randomized, Controlled, Parallel-Group Pre-Post Design – a Study Protocol from Research Consortium OptiMD
    Article Snippet: .. Concentration of MgCl 2 and annealing temperature had been optimized for these three sets of PCR primers and provided best results for 4 mM MgCl 2 and 60° C. A qPCR mixture with 20 μl final reaction volume was prepared by using LightCycler 480 Probes Master kit (Roche Diagnostics, Mannheim, Germany, catalog no. 04707494001) which provides a hot-start reaction mix with FastStart Taq DNA Polymerase designed specifically for detecting DNA targets with hydrolysis probes during qPCR with the LightCycler 480 II system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exploratory Deep Phenotyping of Early Antidepressant Treatment Effects in a Longitudinal, Double-Blind, Randomized, Controlled, Parallel-Group Pre-Post Design – a Study Protocol from Research Consortium OptiMD
    Article Snippet: .. Concentration of MgCl 2 and annealing temperature had been optimized for these three sets of PCR primers and provided best results for 4 mM MgCl 2 and 60° C. A qPCR mixture with 20 μl final reaction volume was prepared by using LightCycler 480 Probes Master kit (Roche Diagnostics, Mannheim, Germany, catalog no. 04707494001) which provides a hot-start reaction mix with FastStart Taq DNA Polymerase designed specifically for detecting DNA targets with hydrolysis probes during qPCR with the LightCycler 480 II system. ..

    Article Title: Unraveling and controlling late-onset neurotoxicity of antisense oligonucleotides through strategic chemical modifications
    Article Snippet: RNA was extracted from cells or tissues with ISOGEN, ISOGEN-LS (Nippon Gene, Tokyo, Japan), or miRNeasy mini kit (QIAGEN, Hilden, Germany). .. Complementary DNA was synthesized with Takara 5× PrimeScript RT master mix (Takara Bio, Shiga, Japan) and quantified by quantitative real-time PCR (real-time qPCR) with LightCycler 480 system (Roche Diagnostics, Rotkreuz, Switzerland) with LightCycler 480 probes master kit (Roche Diagnostics GmbH, Mannheim, Germany) and primers. ..

    Article Title: Utilisation du matériel génétique extrait des tests de diagnostic rapide du paludisme en PCR lors du Grand Magal de Touba au Sénégal
    Article Snippet: .. All real-time quantitative PCR assays were performed using a C1000 TouchTM thermocycler (Bio-Rad, Hercules, CA, USA) with the LightCycler ® 480 Probes Master kit (Roche Diagnostics, France), following the manufacturer’s recommendations. ..

    Article Title: Guanine base modifications in antisense oligonucleotides mitigate acute central nervous system toxicity
    Article Snippet: Complementary DNA (cDNA) was synthesized from the isolated RNA with the Takara 5× PrimeScript RT Master Mix (Takara Bio Inc., Kusatsu, Shiga, Japan). .. Quantitative real-time PCR was carried out with the LightCycler 480 Probes Master kit (Roche Applied Science, Penzberg, Germany) on a LightCycler 480 system (Roche Diagnostics, Mannheim, Germany). .. Primers and probes for mouse Bace1 and Mapt were designed by TaqMan (Applied Biosystems, Foster City, CA, USA), while those for Actb were designed by Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Identification de cas de borréliose à tiques par qPCR à partir de tests rapides de diagnostic du paludisme lors du Grand Magai de Touba au Sénégal
    Article Snippet: We performed all quantitative real-time PCR assays using a C1000 TouchTM thermocycler (Bio-Rad, Hercules, CA, USA) to detect P. falciparum, P. malariae, P. vivax, P. ovale, dengue virus (DENV), C. burnetti, Borrelia spp., and Bartonella spp. (Table ). .. Real-time PCR amplifications were performed using the LightCycler ® 480 Probes Master Kit (Roche Diagnostics, France), following the manufacturer’s instructions. ..

    Article Title: Etiology of diarrhea among children under five years in Thai Binh, Vietnam: a prospective study.
    Article Snippet: The Multiplex RNA Virus Master Kit (Roche Diagnosis, France) was used for detecting hepatitis A and E virus, adenovirus, rotavirus, norovirus, astrovirus, and internal controls MS2 phage using one-step simplex real-time quantitative RT-PCR amplifications. .. Real-time PCR amplifications were performed using the LightCycler® 480 Probes Master kit (Roche Diagnostics, France) according to the manufacturer’s recommendations. ..

    Article Title: Genotype-stratified adjunctive dexamethasone for tuberculous meningitis in HIV-negative adults: a randomized controlled phase 3 trial
    Article Snippet: DNA quality and concentration were determined by Nanodrop 2000 v1.0 Spectrophotometer (Thermo Scientific). rs17525495 was genotyped by Taqman using predesigned assay kits (Applied Biosystems) according to the supplier’s instructions ( https://documents.thermofisher.com/TFS-Assets/LSG/manuals/MAN0009593_TaqManSNP_UG.pdf ). .. We performed real-time PCR with 15 ng of DNA using a LightCycler 480 Probes Master kit on the LightCycler 480 real-time PCR system (Roche) according to the supplier’s protocol ( https://pim-eservices.roche.com/LifeScience/Document/4dd0e207-97ed-e311-98a1-00215a9b0ba8 ). ..

    Synthesized:

    Article Title: Unraveling and controlling late-onset neurotoxicity of antisense oligonucleotides through strategic chemical modifications
    Article Snippet: RNA was extracted from cells or tissues with ISOGEN, ISOGEN-LS (Nippon Gene, Tokyo, Japan), or miRNeasy mini kit (QIAGEN, Hilden, Germany). .. Complementary DNA was synthesized with Takara 5× PrimeScript RT master mix (Takara Bio, Shiga, Japan) and quantified by quantitative real-time PCR (real-time qPCR) with LightCycler 480 system (Roche Diagnostics, Rotkreuz, Switzerland) with LightCycler 480 probes master kit (Roche Diagnostics GmbH, Mannheim, Germany) and primers. ..

    Amplification:

    Article Title: LpxR and PagL expression in live attenuated auxotrophic Pseudomonas aeruginosa vaccines modulates lipid A reactogenicity in vitro while preserving immunogenicity
    Article Snippet: .. Amplification was conducted using the LightCycler ® 480 Probes Master Kit (Roche Diagnostics, Germany), according to the supplier’s instructions. .. Gene-specific primers and optimal TaqMan probes from the Universal Probe Library (Roche, Germany) were used ( ).



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